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Fusaricidins in

Weicheng LIU, Xiaoli WU, Xuelian BAI, Hong ZHANG, Dan DONG, Taotao ZHANG, Huiling WU

《农业科学与工程前沿（英文）》 2018年 第5卷 第2期   页码 262-270 doi: 10.15302/J-FASE-2017166

摘要： Four kinds of antifungal compounds from an extract of A21 with molecular masses of 883.56, 897.59, 947.55, and 961.58 Da were characterized as the members of fusaricidin-type of antibiotics according to LC-MS analysis. Fusaricidins isolated from culture filtrate displayed high antagonistic activity against several plant fungal pathogens, especially , the causal agent of gray mold. The fusaricidins biosynthetic gene cluster (BGC) from A21 was cloned by PCR and comparative cluster analysis revealed that gene , the 3′ boundary of the fusaricidin BGC in strain PKB1, was not present in fusaricidin BGC of A21, indicating that is not necessary for fusaricidin synthesis. Fusaricidin extract from A21 significantly reduced gray mold disease incidence and severity on tomato. The mRNA levels for three patho-genesis-related proteins (PRs) revealed that treatment of tomato leaves with fusaricidin extract induced the expression of PR genes to different levels, suggesting that one reason for the reduction of gray mold infection by fusaricidin is induction of PR proteins, which lead to increased resistance to pathogens. This is the first report of the application of fusaricidins to control tomato gray mold and the comparative cluster analysis provides important molecular basis for research on fusaricidin biosynthesis.

关键词： antifungal activity     biosynthetic gene cluster     Botrytis cinerea     fusaricidin     Paenibacillus polymyxa    

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Triggering the biocontrol of

Kandasamy SARAVANAKUMAR, Zhixiang LU, Hai XIA, Meng WANG, Jianan SUN, Shaoqing WANG, Qiang-qiang WANG, Yaqian LI, Jie CHEN

《农业科学与工程前沿（英文）》 2018年 第5卷 第2期   页码 271-279 doi: 10.15302/J-FASE-2018214

摘要： This study reports a strain of CCTCC-SBW0162 with potential to enhance biocontrol activity against gray mold pathogen, , and with a pivotal role in tomato ( ) plant growth enhancement. A total of 254 isolates were screened by antagonistic assay. Of these, 10 were selected for greenhouse experiments based on their greater inhibition of . The antagonistic assay and greenhouse experiments indicated that CCTCC-SBW0162 gave the highest inhibition rate (90.6%) and disease reduction (80.7%). Also, to study the possible mechanism associated with antifungal activity of CCTCC-SBW0162 against , molecular docking was used to assess the interactions between CCTCC-SBW0162-derived metabolites, and pathogencity and virulence related proteins of . The molecular docking results indicated that the combination of harzianopyridone, harzianolide and anthraquinone C derived from CCTCC-SBW0162 could synergistically improve antifungal activity against through the inhibition/modification of pathogenicity and virulence related proteins. However, this computerized modeling work emphasized the need for further study in the laboratory to confirm the effect derived metabolites against the proteins of and their interactions.

关键词： anthraquinone     Botrytis cinerea     harzianolide     harzianopyridone     molecular docking     Trichoderma harzianum    

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A Novel Light-Responsive Gene BcCfaS Regulates the Photomorphogenesis and Virulence of Botrytis cinerea

Guangjin Li,Zhanquan Zhang,Yong Chen,Tong Chen,Boqiang Li,Shiping Tian,

《工程（英文）》 doi: 10.1016/j.eng.2023.08.015

摘要： Light is a fundamental environmental factor for living organisms on earth—not only as a primary energy source but also as an informational signal. In fungi, light can be used as an indicator for both time and space to control important physiological and morphological responses. Botrytis cinerea (B. cinerea) is a devastating phytopathogenic fungus that exploits light cues to optimize virulence and the balance between conidiation and sclerotia development, thereby improving its dispersal and survival in ecosystems. However, the components and mechanisms underlying these processes remain obscure. Here, we identify a novel light-signaling component in B. cinerea, BcCfaS, which encodes a putative cyclopropane fatty-acyl-phospholipid synthase. BcCfaS is strongly induced by light at the transcriptional level and plays a crucial role in regulating photomorphogenesis. Deletion of BcCfaS results in reduced vegetative growth, altered colony morphology, impaired sclerotial development, and enhanced conidiation in a light-dependent manner. Moreover, the mutant exhibits serious defects in stress response and virulence on the host. Based on a lipidomics analysis, a number of previously unknown fungal lipids and many BcCfaS-regulated lipids are identified in B. cinerea, including several novel phospholipids and fatty acids. Importantly, we find that BcCfaS controls conidiation and sclerotial development by positively regulating methyl jasmonate (MeJA) synthesis to activate the transcription of light-signaling components, revealing for the first time the metabolic base of photomorphogenesis in fungi. Thus, we propose that BcCfaS serves as an integration node for light and lipid metabolism, thereby providing a regulatory mechanism by which fungi adapt their development to a changing light environment. These new findings provide an important target for antifungal design to prevent and control fungal disease.

关键词： Light     Botrytis cinerea     Cyclopropane fatty-acyl-phospholipid synthase     Lipid metabolism     Photomorphogenesis     Virulence    

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IN VITRO ACTIVITY OF EXTRACTS OF FIVE MEDICINAL PLANT SPECIES ON PLANT PATHOGENIC FUNGI

《农业科学与工程前沿（英文）》 2021年 第8卷 第4期   页码 635-644 doi: 10.15302/J-FASE -2020343

摘要：

The antifungal effectiveness of extracts of five medicinal plant species was determined. The inhibitory activity of extracts of Eucalyptus tereticornis, Xanthium sibiricum, Artemisia argyi, Tupistra chinensis and Pyrola calliantha were evaluated against the mycelial growth of the plant pathogenic fungi Aspergillus niger, Botrytis cinerea, Penicillium digitatum, P. expansum, P. italicumand Rhizopus stolonifer. All plant extracts were prepared at 60°C using solvents (either water, 50% ethanol (v/v), 95% ethanol (v/v), ethyl acetate or petroleum ether). Fungicidal effects of all plants tested were confirmed. Different extracts from the same plant species gave different degrees of inhibition. All aqueous extracts had weak or no activity on all fungi tested. Ethyl acetate and 95% ethanol extracts from T. chinensis rhizomes gave greater inhibition and a broader spectrum inhibition than the other extracts. T. chinensis may have potential as a new natural fungicide and may be used for the preservation of agricultural and forestry products such as fruits and vegetables.

 

关键词： Botrytis cinerea / food preservation / plant diseases / plant pathogenic fungi / Tupistra chinensis    

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Transformation of triclosan by a novel cold-adapted laccase from

Yuanyuan Shi, Deyang Kong, Jiayang Liu, Junhe Lu, Xiaoming Yin, Quansuo Zhou

《环境科学与工程前沿（英文）》 2017年 第11卷 第3期 doi: 10.1007/s11783-017-0927-5

摘要： This work investigated the transformation of triclosan (TCS) by laccase produced by a pathogen isolated from rotten tomato. The pathogen was characterized as sp. FQ, belonging to subphylum . The laccase exhibited cold-adaptation with relatively high activity at 20°C. The laccase could effectively transform TCS. Approximately 62% TCS could be removed at dose of 1.0 unit·mL in 120 min. The reaction rate appeared to be to the concentration of the substrate, suggesting the laccase activity remained stable during the reaction. Transformation products of TCS were analyzed by mass spectrometry and it was revealed that TCS dimers were formed via radical coupling pathways. During this process, laccase catalyzed oxidation of TCS to form a radical intermediate is the rate limiting step. However, this step can be reversed by humic acid. Overall, the laccase showed great potential in the treatment of phenolic contaminants. Since laccase is widely presented in natural environment, this study also revealed an important pathway involved in the transformation of phenolic contaminants in the environment.

关键词： Laccase     Botrytis cinerea     Triclosan     Transformation     Kinetics    

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Development of a new method for RMR and Q classification method to optimize support system in tunneling

Asghar RAHMATI,Lohrasb FARAMARZI,Manouchehr SANEI

《结构与土木工程前沿（英文）》 2014年 第8卷 第4期   页码 448-455 doi: 10.1007/s11709-014-0262-x

摘要： Rock mass classification system is very suitable for various engineering design and stability analysis. classification method is confirmed by Japan Highway Public Corporation that this method can figure out either strength or deformability of rock mass, further appropriating the amount of rock bolts, thickness of shotcrete, and size of pitch of steel ribs just after the blasting procedure. Based on these advantages of method, in this study, according to data of five deep and long tunnels in Iran, two equations for estimating the value of method from and classification systems were developed. These equations as a new method were able to optimize the support system for and classification systems. From classification and its application in these case studies, it is pointed out that the method for the design of support systems in underground working is more reliable than the and classification systems.

关键词： JH classification     Q and RMR classification     new method    

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抗白粉病基因 <i>Pm40i> 在我国“后 <i>Pm21i> 时代”小麦育种中的重要作用 Perspective

唐圣雯,胡玉亭,钟胜福,罗培高

《工程（英文）》 2018年 第4卷 第4期   页码 500-506 doi: 10.1016/j.eng.2018.06.004

摘要：

由小麦白粉菌[Blumeria graminis f. sp. tritici（Bgt）] 引起的小麦白粉病是一种重要的小麦叶部病害，对小麦的产量产生很大的影响。20 世纪80 年代，通过簇毛麦（Heuchera villosa）的6VS 染色体与小麦6AL 染色体易位将抗白粉病基因Pm21 转移到普通小麦中。最近，在一些地方发现了对Pm21 有毒的Bgt，虽然这些菌株的病理学特性还有待研究，但这一现象提醒小麦育种者应注意应用Pm21 的风险。来源于普通小麦与中间偃麦草（Thinopyrum intermedium）杂种后代的抗白粉病基因Pm40，被定位在小麦7BS 染色体上，对Bgt 具有广谱和持久的抗性。通过细胞学研究，并未在Pm40 的载体品种中发现大片段外缘染色体。过去几年的研究发现，Pm40 的载体品种具有优良的农艺性状。因此，我们相信在未来的育种工程中，Pm40 将会在Pm21 的抗性被克服之后起巨大的作用。另外，Pm21 和Pm40 都来源于外缘物种，这暗示着外源基因的抗性可能比小麦本身的基因更为持久和有效。

关键词： 小麦     白粉病     <i>Pm21i>     <i>Pm40i>     外源基因     内源抗性    

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ACTIVITY OF EXTRACTS OF FIVE MEDICINAL PLANT SPECIES ON PLANT PATHOGENIC FUNGI

Shufeng YAO, Jiali XU, Haibo ZHANG, Hong GAO, Shibin SHANG, Dan WANG

《农业科学与工程前沿（英文）》 2021年 第8卷 第4期   页码 635-644 doi: 10.15302/J-FASE-2020343

摘要： The antifungal effectiveness of extracts of five medicinal plant species was determined. The inhibitory activity of extracts of , , , and were evaluated against the mycelial growth of the plant pathogenic fungi , , and . All plant extracts were prepared at 60°C using solvents (either water, 50% ethanol ( ), 95% ethanol ( ), ethyl acetate or petroleum ether). Fungicidal effects of all plants tested were confirmed. Different extracts from the same plant species gave different degrees of inhibition. All aqueous extracts had weak or no activity on all fungi tested. Ethyl acetate and 95% ethanol extracts from rhizomes gave greater inhibition and a broader spectrum inhibition than the other extracts. may have potential as a new natural fungicide and may be used for the preservation of agricultural and forestry products such as fruits and vegetables.

关键词： Botrytis cinerea     food preservation     plant diseases     plant pathogenic fungi     Tupistra chinensis    

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NiFeRuO<i>xi> nanosheets on Ni foam as an electrocatalyst for efficient

《化学科学与工程前沿（英文）》 2023年 第17卷 第11期   页码 1698-1706 doi: 10.1007/s11705-023-2334-8

摘要： The electrocatalyst NiFeRuOx/NF, comprised of NiFeRuOx nanosheets grown on Ni foam, was synthesized using a hydrothermal process followed by thermal annealing. NiFeRuOx/NF displays high electrocatalytic activity and stability for overall alkaline seawater splitting: 98 mV@ 10 mA∙cm−2 in hydrogen evolution reaction, 318 mV@ 50 mA∙cm−2 in oxygen evolution reaction, and a cell voltage of 1.53 V@ 10 mA∙cm−2, as well as 20 h of durability. A solar-driven system containing such a bifunctional NiFeRuOx/NF has an almost 100% Faradaic efficiency. The NiFeRuOx coating around Ni foam is an anti-corrosion layer and also a critical factor for enhancement of bifunctional performances.

关键词： NiFeRuOx nanosheets     Ni foam     electrocatalysis     overall seawater splitting     solar-driven system    

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2014—2016年食品动物源RE-<i>cmeABCi>阳性弯曲菌流行与耐药性现状 Article

刘德俊, 刘蔚雯, 李星, 姚红, 沈张奇, 汪洋, 沈建忠

《工程（英文）》 2020年 第6卷 第1期   页码 34-39 doi: 10.1016/j.eng.2019.10.013

摘要：

弯曲菌是引起人类胃肠炎的一类重要食源性致病菌。RE-CmeABC是近年来弯曲菌中新发现的一种增强型耐药外排泵，可介导对兽医和人医临床治疗弯曲菌感染的重要药物，如氟喹诺酮类、酰胺醇类、大环内酯类及四环素类等药物的高水平耐药，而目前尚未有对该外排泵的大范围流行病学调查。因此，本研究调查了2014—2016年连续三年间我国山东、上海和广东等三个重点养殖区食品动物源RE-cmeABC阳性弯曲菌的流行与耐药性现状。结果显示，在1088株弯曲菌（931株结肠弯曲菌与157株空肠弯曲菌）中共检测出122株（11.2%）RE-cmeABC阳性菌株，包括111株（70.7%）空肠弯曲菌与11株（1.2%）结肠弯曲菌，空肠弯曲菌中RE-cmeABC的阳性率显著高于结肠弯曲菌。111株RE-cmeABC阳性空肠弯曲菌与46株RE-cmeABC阴性空肠弯曲菌相比在氟苯尼考、克林霉素和红霉素的耐药率上具有显著差异（P < 0.05），而在环丙沙星、四环素和庆大霉素的耐药率上无显著差异。然而，携带RE-cmeABC的菌株可以使环丙沙星与四环素对弯曲菌的MIC值分布趋于更高水平范围。脉冲场凝胶电泳分型（PFGE）结果显示RE-cmeABC阳性菌株在上海和广东地区以水平传播为主，在山东地区以克隆传播为主；三株分离于上海和广东的阳性菌为同一克隆型，4株分离于上海和山东的阳性菌为同一克隆型。本研究表明RE-cmeABC已在我国食品动物源弯曲菌中广泛流行，对公共卫生安全具有潜在威胁。

关键词： RE- <i> cmeABC i>     弯曲菌     环丙沙星     氟苯尼考    

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A highly efficient methodology for the preparation of

Yongxin Zhang, Shucheng Wang, Yaodong Huang

《化学科学与工程前沿（英文）》 2021年 第15卷 第3期   页码 679-686 doi: 10.1007/s11705-020-1979-9

摘要： A convenient and highly efficient method is described for the synthesis of -methoxycarbazole derivatives, including those with sterically demanding, benzannulated, or strongly electron-donating or -withdrawing substituents. Various -methoxycarbazole derivatives were directly prepared in good-to-moderate yields by the Pd (dba) CHCl /9,9-dimethyl-4,5-bis(diphenylphosphino)xanthene-catalyzed reactions of the corresponding dibromobiphenyl compounds and methoxya-mine. Based on this methodology, the first total synthesis of 3,3′-[oxybis(methylene)]bis(9-methoxy-9 -carbazole), an antimicrobial dimeric carbazole alkaloid previously isolated from the stem bark of , was achieved in 18% yield over seven steps from 1,2-dibromobenzene.

关键词： N-methoxyl carbazole     dimeric N-methoxyl carbazole     alkaloid     total synthesis     double N-arylation of methoxyamine    

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通过<i> B i>位Ta置换对<i> AB i> O4型EuNbO4的热学和力学性质进行优化 Article

陈琳, 冯晶

《工程（英文）》 2020年 第6卷 第2期   页码 177-184 doi: 10.1016/j.eng.2019.12.006

摘要：

铁弹性的ABO4型RETaO4陶瓷和RENbO4陶瓷（RE表示稀土）是具有潜在应用价值的热障涂层（TBC）材料，研究人员正在对其进行大量研究。结果显示， RETaO4陶瓷的力学性质优于RENbO4陶瓷的力学性质。在本研究中，我们通过B位钽（Ta）置换对利用固相法（SSR）制备的EuNbO4陶瓷的热学和力学性质进行优化。我们使用X射线衍射（XRD）法和拉曼光谱技术对所制备的晶体结构进行确认，并利用扫描电子显微镜（SEM）对结构表面的微观形貌进行观察。结果表明，通过B位Ta置换可以有效提高EuNbO4陶瓷的杨氏模量和热膨胀系数（TEC），且其最大值分别为169 GPa和1.12×10−5 K−1（1200 ℃）； EuNbO4陶瓷的热导率被降低至1.52 W·K–1·m–1（700 ℃），且材料的抗热辐射能力得到了改善。我们建立了声子热扩散系数与温度之间的关系，用以通过消除热辐射效应来确定声子本征热导率。研究结果表明，通过B位Ta置换能够有效优化EuNbO4的热学和力学性质，从而使得此种材料在将来可成为一种高温结构陶瓷材料。

关键词： 热障涂层，稀土铌酸盐，置换，热导率，热膨胀系数，杨氏模量    

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Traces and echoes of <i>De Architecturai> by Marcus Vitruvius Pollio in the work of Xu Guangqi in 17th

Michela CIGOLA,Yibing FANG

《机械工程前沿（英文）》 2016年 第11卷 第1期   页码 3-11 doi: 10.1007/s11465-015-0359-5

摘要：

This study aims to investigate the role played by Xu Guangqi (1562–1633), minister of the Ming Dynasty, in the development of European scientific and technical knowledge in China between the 16th and 17th centuries by analyzing a book of Western technology that he wrote, namely, Taixi Shuifa (On Western Hydraulics). Several Western books related to machine knowledge are searched to trace the source of the illustrations in Taixi Shuifa. We found that Archimedes’ screw and Ctesibius’ machine, which are included in Vitruvius’ De Architectura volumes, also appear in the work of Xu Guangqi.

关键词： Vitruvius’ De Architectura     Xu Guangqi’s Taixi Shuifa     Jesuit missionaries     Italian Renaissance     drawing     representation    

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Accounting for the uncertainties in the estimation of average shear wave velocity using – correlations

《结构与土木工程前沿（英文）》 2021年 第15卷 第5期   页码 1199-1208 doi: 10.1007/s11709-021-0749-1

摘要： Site-specific seismic hazard analysis is crucial for designing earthquake resistance structures, particularly in seismically active regions. Shear wave velocity ( V S) is a key parameter in such analysis, although the economy and other factors restrict its direct field measurement in many cases. Various V S–SPT– N correlations are routinely incorporated in seismic hazard analysis to estimate the value of V S. However, many uncertainties question the reliability of these estimated V S values. This paper comes up with a statistical approach to take care of such uncertainties involved in V S calculations. The measured SPT– N values from all the critical boreholes were converted into statistical parameters and passed through various correlations to estimate V S at different depths. The effect of different soil layers in the boreholes on the Vs estimation was also taken into account. Further, the average shear wave velocity of the top 30 m soil cover ( V S30) is estimated after accounting for various epistemic and aleatoric uncertainties. The scattering nature of the V S values estimated using different V S– N correlations was reduced significantly with the application of the methodology. Study results further clearly demonstrated the potential of the approach to eliminate various uncertainties involved in the estimation of V S30 using general and soil-specific correlations.

关键词： uncertainties     V S– N correlations     V S30     SPT data     statistical methodology    

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<i>RR1i> and <i>RR2i> gene deletion affects the immunogenicity of a live attenuated pseudorabies

Shijun YAN,He YAN,Chaolin ZHANG,Tongyan WANG,Qingyuan YANG,Zhe SUN,Yan XIAO,Feifei TAN,Xiangdong LI,Kegong TIAN

《农业科学与工程前沿（英文）》 2016年 第3卷 第1期   页码 81-86 doi: 10.15302/J-FASE-2016088

摘要： As virulence-determining genes, and encode the small subunit and large subunit of viral ribonucleotide reductase (RR) in pseudorabies virus which have been extensively studied in mice. However, their role in pigs has not been adequately investigated. In this study, we deleted and genes based on a / / triple gene-deleted pseudorabies virus and tested its efficacy in pigs as a vaccine candidate. The rescued virus showed similar growth properties and plaque size as its parent strain. In an animal study, the virus could elicit humoral immune responses shown by generation of gB-specific antibodies and virus neutralizing antibodies. However, vaccination could not provide protection against virulent pseudorabies virus challenge since vaccinated pigs showed clinical pseudorabies-specific syndromes. The deficiency in protection may due to the generation of late and low levels of gB antibodies and virus neutralizing antibodies.

关键词： pseudorabies virus     RR1 and RR2     ribonucleotide reductase     vaccine candidate    

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标题 作者 时间 类型 操作

Fusaricidins in

Weicheng LIU, Xiaoli WU, Xuelian BAI, Hong ZHANG, Dan DONG, Taotao ZHANG, Huiling WU

期刊论文

Triggering the biocontrol of

Kandasamy SARAVANAKUMAR, Zhixiang LU, Hai XIA, Meng WANG, Jianan SUN, Shaoqing WANG, Qiang-qiang WANG, Yaqian LI, Jie CHEN

期刊论文

A Novel Light-Responsive Gene BcCfaS Regulates the Photomorphogenesis and Virulence of Botrytis cinerea

Guangjin Li,Zhanquan Zhang,Yong Chen,Tong Chen,Boqiang Li,Shiping Tian,

期刊论文

IN VITRO ACTIVITY OF EXTRACTS OF FIVE MEDICINAL PLANT SPECIES ON PLANT PATHOGENIC FUNGI

期刊论文

Transformation of triclosan by a novel cold-adapted laccase from

Yuanyuan Shi, Deyang Kong, Jiayang Liu, Junhe Lu, Xiaoming Yin, Quansuo Zhou

期刊论文

Development of a new method for RMR and Q classification method to optimize support system in tunneling

Asghar RAHMATI,Lohrasb FARAMARZI,Manouchehr SANEI

期刊论文

抗白粉病基因 <i>Pm40i> 在我国“后 <i>Pm21i> 时代”小麦育种中的重要作用

唐圣雯,胡玉亭,钟胜福,罗培高

期刊论文

ACTIVITY OF EXTRACTS OF FIVE MEDICINAL PLANT SPECIES ON PLANT PATHOGENIC FUNGI

Shufeng YAO, Jiali XU, Haibo ZHANG, Hong GAO, Shibin SHANG, Dan WANG

期刊论文

NiFeRuO<i>xi> nanosheets on Ni foam as an electrocatalyst for efficient

期刊论文

2014—2016年食品动物源RE-<i>cmeABCi>阳性弯曲菌流行与耐药性现状

刘德俊, 刘蔚雯, 李星, 姚红, 沈张奇, 汪洋, 沈建忠

期刊论文

A highly efficient methodology for the preparation of

Yongxin Zhang, Shucheng Wang, Yaodong Huang

期刊论文

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